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IFN Gamma Polyclonal antibody

IFN Gamma Polyclonal Antibody for IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat No : 30293-1-AP

Print datasheet

Synonyms

IFN γ, Ifng, IFNG2, IFN-Gamma, IFN-γ, interferon gamma

验证数据展示

  • IHC staining of rat spleen using 30293-1-AP

    IHC staining of rat spleen using 30293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 30293-1-AP

    IHC staining of rat spleen using 30293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of rat splenocytes using 30293-1-AP

    FC experiment of rat splenocytes using 30293-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IFN Gamma (30293-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35), and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPMA and ionomycin treated rat splenocytes cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30293-1-AP targets IFN Gamma in IHC, ELISA, FC (Intra) applications and shows reactivity with rat samples.

Tested Applications IHC, ELISA, FC (Intra) Application Description
Tested Reactivity rat
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon gamma
Synonyms IFN γ, Ifng, IFNG2, IFN-Gamma, IFN-γ, interferon gamma
Calculated Molecular Weight18 kDa
GenBank Accession NumberNM_138880
Gene Symbol IFN-gamma
Gene ID (NCBI) 25712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

实验方案

Product Specific Protocols
IHC protocol for IFN Gamma antibody 30293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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