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IFN-gamma Polyclonal antibody

IFN-gamma Polyclonal Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30293-1-AP

Print datasheet

Synonyms

IFN Gamma, Ifng, interferon gamma, IFN-γ, IFN-gamma,IFN gamma,IFN γ,IFNγ

验证数据展示

  • IHC staining of rat spleen using 30293-1-AP

    IHC staining of rat spleen using 30293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 30293-1-AP

    IHC staining of rat spleen using 30293-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of rat splenocytes using 30293-1-AP

    FC experiment of rat splenocytes using 30293-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IFN Gamma (30293-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35), and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPMA and ionomycin treated rat splenocytes cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30293-1-AP targets IFN-gamma in IHC, FC (Intra), ELISA applications and shows reactivity with rat samples.

Tested Applications IHC, FC (Intra), ELISA Application Description
Tested Reactivity rat
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon gamma
Synonyms IFN Gamma, Ifng, interferon gamma, IFN-γ, IFN-gamma,IFN gamma,IFN γ,IFNγ
Calculated Molecular Weight18 kDa
GenBank Accession NumberNM_138880
Gene Symbol IFN-gamma
Gene ID (NCBI) 25712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

实验方案

Product Specific Protocols
IHC protocol for IFN-gamma antibody 30293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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