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验证数据展示

  • FC experiment of wistar rat splenocytes using 98001-1-RR

    FC experiment of wistar rat splenocytes using 98001-1-RR

    1X10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Wistar rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-RR, Clone: 6K20) and PE-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody. Cells were co-stained with 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98001-1-RR

    Affinity and Kinetic Characterization of 98001-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98001-1-RR against Rat IFN-gamma were performed. The affinity constant is below 1 pM.

Anti-Rat IFN-gamma Rabbit Recombinant Antibody

IFN-gamma Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98001-1-RR

产品说明书

CloneNo. 6K20

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

FC (Intra)

别名

IFN Gamma, Ifng, interferon gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNG2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inConA, PMA and ionomycin stimulated Wistar rat splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98001-1-RR targets IFN-gamma in FC (Intra) applications and shows reactivity with rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon gamma
别名 IFN Gamma, Ifng, interferon gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNG2
计算分子量18 kDa
GenBank蛋白编号NM_138880
基因名称 IFN-gamma
Gene ID (NCBI) 25712
RRIDAB_3672145
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01581
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

实验方案

Product Specific Protocols
FC protocol for IFN-gamma antibody 98001-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols