• Featured Product
  • KD/KO Validated

IFN-gamma R1 Polyclonal antibody

IFN-gamma R1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10808-1-AP

Print datasheet

Synonyms

IFNGR1, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN gamma receptor 1



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10808-1-AP targets IFN-gamma R1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen IFN-gamma R1 fusion protein Ag1114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon gamma receptor 1
Synonyms IFNGR1, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN gamma receptor 1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC005333
Gene Symbol IFNGR1
Gene ID (NCBI) 3459
RRIDAB_2121604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

实验方案

Product Specific Protocols
WB protocol for IFN-gamma R1 antibody 10808-1-APDownload protocol
IHC protocol for IFN-gamma R1 antibody 10808-1-APDownload protocol
IP protocol for IFN-gamma R1 antibody 10808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cancer Discov

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Authors - Wan Du
mouseWB

Nat Commun

DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.

Authors - Baiwen Chen
humanWB

Dev Cell

Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells.

Authors - Elena Parmigiani
human,mouseWB

Oncogene

Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia

Authors - Xiaoling Xie
  • KD Validated
mouseWB,IHC

Ecotoxicol Environ Saf

Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts

Authors - Jiaqi Tian
humanIHC

J Neuropathol Exp Neurol

Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.

Authors - Rodriguez Fausto J FJ