IFNGR2 Polyclonal antibody

IFNGR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10266-1-AP

Print datasheet

Synonyms

AF 1, IFGR2, IFN gamma R2, IFN gamma receptor 2, IFNGR2, IFNGT1, Interferon gamma receptor 2, Interferon gamma transducer 1



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10266-1-AP targets IFNGR2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen IFNGR2 fusion protein Ag0317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon gamma receptor 2 (interferon gamma transducer 1)
Synonyms AF 1, IFGR2, IFN gamma R2, IFN gamma receptor 2, IFNGR2, IFNGT1, Interferon gamma receptor 2, Interferon gamma transducer 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC003624
Gene Symbol IFNGR2
Gene ID (NCBI) 3460
RRIDAB_2121709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit which attaches to IFN-γ with a significantly lower affinity. Two subunits bind one IFN-γ dimer. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

实验方案

Product Specific Protocols
WB protocol for IFNGR2 antibody 10266-1-APDownload protocol
IHC protocol for IFNGR2 antibody 10266-1-APDownload protocol
IP protocol for IFNGR2 antibody 10266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Science

Aberrant type 1 immunity drives susceptibility to mucosal fungal infections.

Authors - Timothy J Break
mouseWB

Nat Commun

DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.

Authors - Baiwen Chen
humanWB

Cancer Commun (Lond)

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

Authors - Qingnan Wu
humanWB

PLoS One

Impact of adipose tissue or umbilical cord derived mesenchymal stem cells on the immunogenicity of human cord blood derived endothelial progenitor cells.

Authors - Kefang Tan
humanWB

Biomol Biomed

Identification of RUNX1 and IFNGR2 as prognostic-related biomarkers correlated with immune infiltration and subtype differentiation of low-grade glioma

Authors -
humanIF,WB

Sci Adv

Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling

Authors - Benjamin Ledoux