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IFRD1 Polyclonal antibody

IFRD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12939-1-AP

Print datasheet

Synonyms

Nerve growth factor-inducible protein PC4, Interferon-related developmental regulator 1

验证数据展示

  • WB analysis of HeLa using 12939-1-AP

    WB analysis of HeLa using 12939-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12939-1-AP (IFRD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12939-1-AP (IFRD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12939-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12939-1-AP (IFRD1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, human skeletal muscle tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12939-1-AP targets IFRD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IFRD1 fusion protein Ag3988 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-related developmental regulator 1
Synonyms Nerve growth factor-inducible protein PC4, Interferon-related developmental regulator 1
Calculated Molecular Weight 451 aa, 50 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC001272
Gene Symbol IFRD1
Gene ID (NCBI) 3475
RRIDAB_10597099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFRD1 antibody 12939-1-APDownload protocol
IHC protocol for IFRD1 antibody 12939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

Translation of 5' leaders is pervasive in genes resistant to eIF2 repression.

Authors - Dmitry E Andreev
View Article
humanWB

RNA Biol

Divergent effects of translation termination factor eRF3A and nonsense-mediated mRNA decay factor UPF1 on the expression of uORF carrying mRNAs and ribosome protein genes.

Authors - Affaf Aliouat
View Article