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IFRD2 Polyclonal antibody

IFRD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26952-1-AP

Print datasheet

Synonyms

Interferon-related developmental regulator 2, IFNRP

验证数据展示

  • IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HL-60 using 26952-1-AP

    WB analysis of HL-60 using 26952-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26952-1-AP targets IFRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitybovine
Immunogen IFRD2 fusion protein Ag25057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-related developmental regulator 2
Synonyms Interferon-related developmental regulator 2, IFNRP
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC007265
Gene Symbol IFRD2
Gene ID (NCBI) 7866
RRIDAB_2880700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12894
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFRD2, or interferon-related developmental regulator 2, is a protein that has been identified as a novel factor capable of translationally inactivating ribosomes. It plays a role in regulating gene expression by directly binding to 80S ribosomes, which precludes the binding of mRNA and P-site tRNA, thereby inhibiting translation

实验方案

Product Specific Protocols
WB protocol for IFRD2 antibody 26952-1-APDownload protocol
IHC protocol for IFRD2 antibody 26952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB,IF

J Muscle Res Cell Motil

IFRD2, a target of miR-2400, regulates myogenic differentiation of bovine skeletal muscle satellite cells via decreased phosphorylation of ERK1/2 proteins

Authors - Zhian Gong
View Article