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IFT172 Polyclonal antibody

IFT172 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28441-1-AP

Print datasheet

Synonyms

DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim

验证数据展示

  • WB analysis using 28441-1-AP

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of ARPE-19 using 28441-1-AP

    IF Staining of ARPE-19 using 28441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using IFT172 antibody (28441-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inARPE-19 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28441-1-AP targets IFT172 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity mouse, rat, human
Immunogen IFT172 fusion protein Ag28921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Synonyms DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim
Calculated Molecular Weight 1749 aa, 198 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC137126
Gene Symbol IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UG01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFT172 antibody 28441-1-APDownload protocol
IHC protocol for IFT172 antibody 28441-1-APDownload protocol
IF protocol for IFT172 antibody 28441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols