验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27334-1-AP targets IFT74 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | IFT74 fusion protein Ag26385 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intraflagellar transport 74 homolog (Chlamydomonas) |
| 别名 | CCDC2, CMG 1, CMG1, IFT74 |
| 计算分子量 | 600 aa, 69 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC107742 |
| 基因名称 | IFT74 |
| Gene ID (NCBI) | 80173 |
| RRID | AB_2880847 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LB3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT74 antibody 27334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genet Med Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.
| ||
Pulm Circ Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia | ||
Hum Reprod Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility | ||
Hum Mol Genet Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations | ||
EMBO J The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport
| ||
EMBO Mol Med IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect |
