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  • KD/KO Validated

IFT74 Polyclonal antibody

IFT74 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 27334-1-AP

Print datasheet

Synonyms

CCDC2, CMG 1, CMG1, IFT74



经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27334-1-AP is IFT74 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen IFT74 fusion protein Ag26385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 74 homolog (Chlamydomonas)
Synonyms CCDC2, CMG 1, CMG1, IFT74
Calculated Molecular Weight 600 aa, 69 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC107742
Gene Symbol IFT74
Gene ID (NCBI) 80173
RRIDAB_2880847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LB3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFT74 antibody 27334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Genet Med

Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.

Authors - Minna Luo
  • KD Validated
humanWB

Pulm Circ

Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia

Authors - Saejeong Park
human,mouseIF

Hum Reprod

Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility

Authors - Qi Li
humanWB,IP

Hum Mol Genet

Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations

Authors - Mahmoud R Fassad
humanWB

EMBO J

The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport

Authors - Niels Boegholm
  • KO Validated
mouseWB,CoIP

EMBO Mol Med

IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect

Authors - Jing Dai