- Featured Product
- KD/KO Validated
IFT74 Polyclonal antibody
IFT74 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 27334-1-AP is IFT74 Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB, IP, IF, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | IFT74 fusion protein Ag26385 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | intraflagellar transport 74 homolog (Chlamydomonas) |
Synonyms | CCDC2, CMG 1, CMG1, IFT74 |
Calculated Molecular Weight | 600 aa, 69 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC107742 |
Gene Symbol | IFT74 |
Gene ID (NCBI) | 80173 |
RRID | AB_2880847 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96LB3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for IFT74 antibody 27334-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Genet Med Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.
| ||
Pulm Circ Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia | ||
Hum Reprod Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility | ||
Hum Mol Genet Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations | ||
EMBO J The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport
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EMBO Mol Med IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect |