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验证数据展示

  • WB analysis of K-562 using 60256-1-Ig

    WB analysis of K-562 using 60256-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60256-1-Ig (IGBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 60256-1-Ig

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 60256-1-Ig

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

IGBP1 Monoclonal antibody

IGBP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60256-1-Ig

产品说明书

CloneNo. 2D11A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60256-1-Ig targets IGBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 IGBP1 fusion protein Ag6782 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin (CD79A) binding protein 1
别名 ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4
计算分子量 39 kDa
观测分子量 40 kDa
GenBank蛋白编号BC004137
基因名称 IGBP1
Gene ID (NCBI) 3476
RRIDAB_2881377
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP78318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

实验方案

Product Specific Protocols
WB protocol for IGBP1 antibody 60256-1-IgDownload protocol
IHC protocol for IGBP1 antibody 60256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols