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IGBP1 Monoclonal antibody

IGBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11A8

Cat no : 60256-1-Ig

Print datasheet

Synonyms

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

验证数据展示

  • WB analysis of K-562 using 60256-1-Ig

    WB analysis of K-562 using 60256-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60256-1-Ig (IGBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 60256-1-Ig

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 60256-1-Ig

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60256-1-Ig targets IGBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IGBP1 fusion protein Ag6782 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin (CD79A) binding protein 1
Synonyms ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC004137
Gene Symbol IGBP1
Gene ID (NCBI) 3476
RRIDAB_2881377
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

实验方案

Product Specific Protocols
WB protocol for IGBP1 antibody 60256-1-IgDownload protocol
IHC protocol for IGBP1 antibody 60256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols