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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

    WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF1R antibody included
  • IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF1R antibody included

IGF1R Monoclonal antibody, PBS Only

IGF1R Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66283-1-PBS
CloneNo. 3C6A11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, IGF I receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IGF1R Ready-To-Use IHC Kit. IGF1R primary antibody included.

产品信息

66283-1-PBS targets IGF1R in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name IGF I receptor
Synonyms IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, IGF I receptor
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberNM_000875
Gene Symbol IGF1R
Gene ID (NCBI) 3480
RRIDAB_2881666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08069
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.