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- KD/KO Validated
IGF2BP2 Polyclonal antibody
IGF2BP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (3)
Conjugate
Unconjugated
141
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, human kidney tissue, human heart tissue, COLO 320 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human pancreas cancer tissue, human colon cancer tissue, mouse stomach tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11601-1-AP targets IGF2BP2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | IGF2BP2 fusion protein Ag2142 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | insulin-like growth factor 2 mRNA binding protein 2 |
Synonyms | IGF II mRNA binding protein 2, IGF2 mRNA binding protein 2, IGF2BP2, IMP 2, IMP2, p62, VICKZ family member 2, VICKZ2 |
Calculated Molecular Weight | 594 aa, 65 kDa |
Observed Molecular Weight | 55-65 kDa |
GenBank Accession Number | BC021290 |
Gene Symbol | IGF2BP2 |
Gene ID (NCBI) | 10644 |
RRID | AB_2122672 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6M1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IGF2BP2 antibody 11601-1-AP | Download protocol |
IHC protocol for IGF2BP2 antibody 11601-1-AP | Download protocol |
IP protocol for IGF2BP2 antibody 11601-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
| ||
Nat Cell Biol N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition. | ||
Mol Cell m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3
|