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IGF2BP2 Recombinant antibody

IGF2BP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

6L8

Cat no : 82757-2-RR

Print datasheet

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

验证数据展示

  • WB analysis using 82757-2-RR

    WB analysis using 82757-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 82757-2-RR

    IP experiment of HEK-293 using 82757-2-RR

    IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug.

  • IHC staining of mouse kidney using 82757-2-RR

    IHC staining of mouse kidney using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IGF2BP2 antibody included
  • IHC staining of mouse kidney using 82757-2-RR

    IHC staining of mouse kidney using 82757-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IGF2BP2 antibody included
  • Affinity and Kinetic Characterization of 82757-2-RR

    Affinity and Kinetic Characterization of 82757-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGF2BP2 Ready-To-Use IHC Kit. IGF2BP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82757-2-RR targets IGF2BP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IGF2BP2 fusion protein Ag2142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor 2 mRNA binding protein 2
Synonyms insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2
Calculated Molecular Weight 594 aa, 65 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC021290
Gene Symbol IGF2BP2
Gene ID (NCBI) 10644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

实验方案

Product Specific Protocols
WB protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
IHC protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
IP protocol for IGF2BP2 antibody 82757-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols