IGFBP1 Polyclonal antibody

IGFBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13981-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

AFBP, hIGFBP 1, IBP 1, IBP1, IGF binding protein 1, IGF BP25, IGFBP 1, IGFBP1, Placental protein 12, PP12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, human placenta
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGFBP1 Ready-To-Use IHC Kit. IGFBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13981-1-AP targets IGFBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 IGFBP1 fusion protein Ag5026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IGF binding protein 1
别名 AFBP, hIGFBP 1, IBP 1, IBP1, IGF binding protein 1, IGF BP25, IGFBP 1, IGFBP1, Placental protein 12, PP12
计算分子量 28 kDa
观测分子量 28-35 kDa
GenBank蛋白编号BC057806
基因名称 IGFBP1
Gene ID (NCBI) 3484
RRIDAB_2233481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08833
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

实验方案

Product Specific Protocols
WB protocol for IGFBP1 antibody 13981-1-APDownload protocol
IHC protocol for IGFBP1 antibody 13981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB, IHC

Lung Cancer

Molecular profiling of thymoma with myasthenia gravis: Risk factors of developing myasthenia gravis in thymoma patients.

Authors - Ming-Ching Lee
humanWB

Phytomedicine

Effects of natural 24-epibrassinolide on inducing apoptosis and restricting metabolism in hepatocarcinoma cells

Authors - Hongfei Zhou
humanWB

Biol Reprod

IGFBP1 Involved in the Decreased Birth Weight Due to Fetal High Estrogen Exposure in Mice.

Authors - Min Jin
humanWB

Reprod Biol Endocrinol

Increased expression of prokineticin 2 and its receptor in endometrium of recurrent implantation failure patients decreased the expression of MMP9 important for decidualization.

Authors - Jun Zhai
humanWB

Reprod Biol Endocrinol

PMID: 29925391

Authors - Cheng Li
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