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验证数据展示

  • WB analysis using 31335-1-AP

    WB analysis using 31335-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 31335-1-AP (IGFBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IGFBP1 Polyclonal antibody

IGFBP1 Polyclonal Antibody for WB, ELISA
Cat No. 31335-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

IGF binding protein 1, IBP-1, IBP1, IBP 1, hIGFBP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP1 Ready-To-Use IHC Kit. IGFBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31335-1-AP targets IGFBP1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IGF binding protein 1
别名 IGF binding protein 1, IBP-1, IBP1, IBP 1, hIGFBP 1
计算分子量28 kDa
观测分子量28-35 kDa
GenBank蛋白编号NM_000596.4
基因名称 IGFBP1
Gene ID (NCBI) 3484
RRIDAB_3669949
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08833
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

实验方案

Product Specific Protocols
WB protocol for IGFBP1 antibody 31335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols