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IGFBP1 Recombinant antibody

IGFBP1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240263B9

Cat No : 83347-5-RR

Print datasheet

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

验证数据展示

  • WB analysis of HepG2 using 83347-5-RR

    WB analysis of HepG2 using 83347-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83347-5-RR (IGFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83347-5-RR

    Affinity and Kinetic Characterization of 83347-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83347-5-RR against Human IGFBP1 were performed. The affinity constant is 40.6 nM.

经过测试的应用

Positive WB detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP1 Ready-To-Use IHC Kit. IGFBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83347-5-RR is IGFBP1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IGF binding protein 1
Synonyms IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP
Calculated Molecular Weight28 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberNM_000596.4
Gene Symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08833
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

实验方案

Product Specific Protocols
WB protocol for IGFBP1 antibody 83347-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols