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验证数据展示

  • WB analysis of MCF-7 using 15699-1-AP

    WB analysis of MCF-7 using 15699-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 15699-1-AP

    IHC staining of human pancreas cancer using 15699-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IGFBP2 antibody included
  • IF Staining of A549 using 15699-1-AP

    IF Staining of A549 using 15699-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IGFBP2 antibody (15699-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 15699-1-AP

    IF Staining of HeLa using 15699-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP2 antibody (15699-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15699-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15699-1-AP targets IGFBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 IGFBP2 fusion protein Ag8327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IGF binding protein 2
别名 IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2
计算分子量 36 kDa
观测分子量 32 kDa
GenBank蛋白编号BC004312
基因名称 IGFBP2
Gene ID (NCBI) 3485
RRIDAB_10641053
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18065
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 15699-1-APDownload protocol
IHC protocol for IGFBP2 antibody 15699-1-APDownload protocol
IF protocol for IGFBP2 antibody 15699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols