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验证数据展示

  • WB analysis using 31248-1-AP

    WB analysis using 31248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31248-1-AP (IGFBP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31248-1-AP

    IHC staining of human ovary cancer using 31248-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31248-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IGFBP2 antibody included

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31248-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, SH-SY5Y cells, human plasma
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31248-1-AP targets IGFBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IGF binding protein 2
别名 IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2
计算分子量35 kDa
观测分子量35 kDa
GenBank蛋白编号NM_000597.3
基因名称 IGFBP2
Gene ID (NCBI) 3485
RRIDAB_3669917
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18065
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 31248-1-APDownload protocol
IHC protocol for IGFBP2 antibody 31248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols