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IGFBP2 Recombinant antibody

IGFBP2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240227D4

Cat No : 83283-3-RR

Print datasheet

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

验证数据展示

  • WB analysis using 83283-3-RR

    WB analysis using 83283-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83283-3-RR (IGFBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity experiments of 83283-3-RR

    Affinity experiments of 83283-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83283-3-RR against Human IGFBP2 were performed. The affinity constant is 1.85 nM.

经过测试的应用

Positive WB detected inSH-SY5Y cells, T-47D cells, human plasma sample
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83283-3-RR is IGFBP2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IGF binding protein 2
Synonyms BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2
Calculated Molecular Weight35 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberNM_000597.3
Gene Symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kDa IGFBP protein with degraded fragments of 24 kDa, 19 kDa, and 15 kDa. The levels of IGFBP2 are elevated during the progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 83283-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols