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IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30593-1-AP

Print datasheet

Synonyms

AI649005, IGFBP 3, Igfbp3, IGgfbp3

验证数据展示

  • WB analysis using 30593-1-AP

    WB analysis using 30593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30593-1-AP (IGFBP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30593-1-AP

    IF Staining of A431 using 30593-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IGFBP3 antibody (30593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, mouse colon tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30593-1-AP targets IGFBP3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name insulin-like growth factor binding protein 3
Synonyms AI649005, IGFBP 3, Igfbp3, IGgfbp3
Calculated Molecular Weight32 kDa
Observed Molecular Weight31-45 kDa
GenBank Accession NumberNM_008343
Gene Symbol Igfbp3
Gene ID (NCBI) 16009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

实验方案

Product Specific Protocols
WB protocol for IGFBP3 antibody 30593-1-APDownload protocol
IF protocol for IGFBP3 antibody 30593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols