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IGFBP4 Recombinant antibody, PBS Only (Detector)

IGFBP4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230543B11

Cat no : 83200-1-PBS

Print datasheet

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

验证数据展示

  • Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2, Human IGFBP4 Recombinant Matched Antibody Pair - PBS only. 83200-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0825. 83200-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean IGFBP4 concentration was determined to be 181.3 ng/mL in A549 cell supernatant.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    Serum of sixteen individual healthy human donors was measured. The IGFBP4 concentration of detected samples was determined to be 36.9 ng/mL with a range of 4.7 - 129.3 ng/mL.

  • Cytometric bead array standard curve of MP00279-1

    Cytometric bead array standard curve of MP00279-1

    Cytometric bead array standard curve of MP00279-1, Human IGFBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83200-2-PBS. Detection antibody: 83200-1-PBS. Standard: Eg0825. Range: 1.56-50 ng/mL.

产品信息

83200-1-PBS targets IGFBP4 as part of a matched antibody pair:

MP00279-1: 83200-2-PBS capture and 83200-1-PBS detection (validated in Cytometric bead array)

MP00279-2: 83200-4-PBS capture and 83200-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name insulin-like growth factor binding protein 4
Synonyms IBP 4, HT29 IGFBP, Deb2, BP4, BP 4
Calculated Molecular Weight28 kDa
GenBank Accession NumberNM_001552.3
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22692-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C