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验证数据展示

  • WB analysis using 68512-1-Ig

    WB analysis using 68512-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68512-1-Ig (IGFBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68512-1-Ig

    IHC staining of mouse brain using 68512-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IGFBP5 Monoclonal antibody

IGFBP5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68512-1-Ig
CloneNo. 1C5E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, SKOV-3 cells, Saos-2 cells, U2OS cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68512-1-Ig targets IGFBP5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen IGFBP5 fusion protein Ag33797 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name insulin-like growth factor binding protein 5
Synonyms IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5
Calculated Molecular Weight 31 kDa
Observed Molecular Weight30-33 kDa
GenBank Accession NumberBC011453
Gene Symbol IGFBP5
Gene ID (NCBI) 3488
RRIDAB_3085221
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24593
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

实验方案

Product Specific Protocols
WB protocol for IGFBP5 antibody 68512-1-IgDownload protocol
IHC protocol for IGFBP5 antibody 68512-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols