• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IGFN1 Polyclonal antibody

IGFN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25095-1-AP

Print datasheet

Synonyms

KYIP1, EEF1A2BP1

验证数据展示

  • WB analysis using 25095-1-AP

    WB analysis using 25095-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25095-1-AP (IGFN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25095-1-AP targets IGFN1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IGFN1 fusion protein Ag18731 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin-like and fibronectin type III domain containing 1
Synonyms KYIP1, EEF1A2BP1
Calculated Molecular Weight 1251 aa, 138 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC131529
Gene Symbol IGFN1
Gene ID (NCBI) 91156
RRIDAB_3669462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-like and fibronectin type III domain-containing protein 1 (IGFN1), also known as EEF1A2-binding protein 1 (EEF1A2BP1) or KY-interacting protein 1 (KYIP1), presents structural similarity to sarcomeric proteins belonging to the intracellular Ig superfamily, including myosin-binding protein C fast and slow-type skeletal muscle isoforms (PMID: 18756455). IGFN1 along with KY and FLNC is the part of a Z-band associated protein complex likely providing structural support to the skeletal muscle (PMID: 20206623). IGFN1 gene has been associated with some cancers (PMID: 28027327; 30335789; 35456975). At least five isoforms of IGFN1 produced by alternative splicing have been identified (PMID: 20206623).

实验方案

Product Specific Protocols
WB protocol for IGFN1 antibody 25095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols