验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human lung tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive FC (Intra) detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy Human IgA Ready-To-Use IHC Kit. Human IgA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11449-1-AP targets Human IgA Heavy Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | Human IgA Heavy Chain fusion protein Ag2007 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant alpha 1 |
| 别名 | Human IgA, IGHA1, IGHA 2, IGHA 1, Igh2 |
| 计算分子量 | 496 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC016369 |
| 基因名称 | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_833092 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| IHC protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| IF protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Infect Immune responses and residual SARS-CoV-2 in two critically ill COVID-19 patients before and after lung transplantation. | ||
Cell Immunol Mesenchymal stem cell transplantation worsens intestinal inflammation and microenvironment in PI3Kγ-knockout mice | ||
Sci Rep A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer | ||
Int Immunopharmacol Altered microbial community structure in PI3Kγ knockout mice with colitis impeding relief of inflammation: Establishment of new indices for intestinal microbial disorder. | ||
Evid Based Complement Alternat Med Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress. |
