Human IgA Heavy Chain Polyclonal antibody
Human IgA Heavy Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue, human lung tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive FC (Intra) detected in | Ramos cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11449-1-AP targets Human IgA Heavy Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse, pig |
Immunogen | Human IgA Heavy Chain fusion protein Ag2007 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | immunoglobulin heavy constant alpha 1 |
Synonyms | Human IgA, IGHA1, IGHA 2, IGHA 1, Igh2 |
Calculated Molecular Weight | 496 aa, 53 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC016369 |
Gene Symbol | Human IgA Heavy Chain |
Gene ID (NCBI) | 3493 |
RRID | AB_833092 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
IHC protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
IF protocol for Human IgA Heavy Chain antibody 11449-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Infect Immune responses and residual SARS-CoV-2 in two critically ill COVID-19 patients before and after lung transplantation. | ||
Cell Immunol Mesenchymal stem cell transplantation worsens intestinal inflammation and microenvironment in PI3Kγ-knockout mice | ||
Sci Rep A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer | ||
Int Immunopharmacol Altered microbial community structure in PI3Kγ knockout mice with colitis impeding relief of inflammation: Establishment of new indices for intestinal microbial disorder. | ||
Evid Based Complement Alternat Med Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress. |