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human IgD Polyclonal antibody

human IgD Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16489-1-AP

Print datasheet

Synonyms

IGHD

验证数据展示

  • WB analysis using 16489-1-AP

    WB analysis using 16489-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 16489-1-AP (human IgD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 16489-1-AP

    IF Staining of human tonsillitis using 16489-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using human IgD antibody (16489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:3200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16489-1-AP targets human IgD in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Immunogen human IgD fusion protein Ag9614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin heavy constant delta
Synonyms IGHD
Calculated Molecular Weight 573 aa, 63 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2878264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~70 kDa) of IgD.

实验方案

Product Specific Protocols
WB protocol for human IgD antibody 16489-1-APDownload protocol
IHC protocol for human IgD antibody 16489-1-APDownload protocol
IF protocol for human IgD antibody 16489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Ther

Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer

Authors - Timothy J Smith
View Article