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验证数据展示

  • WB analysis using 16489-1-AP

    WB analysis using 16489-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 16489-1-AP (human IgD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 16489-1-AP

    IF Staining of human tonsillitis using 16489-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using human IgD antibody (16489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

human IgD Polyclonal antibody

human IgD Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 16489-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

IGHD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:3200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16489-1-AP targets human IgD in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human
免疫原 human IgD fusion protein Ag9614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin heavy constant delta
别名 IGHD
计算分子量 573 aa, 63 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC021276
基因名称 IGHD
Gene ID (NCBI) 3495
RRIDAB_2878264
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~70 kDa) of IgD.

实验方案

Product Specific Protocols
WB protocol for human IgD antibody 16489-1-APDownload protocol
IHC protocol for human IgD antibody 16489-1-APDownload protocol
IF protocol for human IgD antibody 16489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Mol Ther

Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transfer

Authors - Timothy J Smith
View Article