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IGHMBP2 Polyclonal antibody

IGHMBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23945-1-AP

Print datasheet

Synonyms

ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2

验证数据展示

  • WB analysis using 23945-1-AP

    WB analysis using 23945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23945-1-AP

    IHC staining of human liver cancer using 23945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with IGHMBP2 antibody included

经过测试的应用

Positive WB detected inHL-60 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGHMBP2 Ready-To-Use IHC Kit. IGHMBP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23945-1-AP is IGHMBP2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IGHMBP2 fusion protein Ag21057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin mu binding protein 2
Synonyms ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2
Calculated Molecular Weight 993 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC105088
Gene Symbol IGHMBP2
Gene ID (NCBI) 3508
RRIDAB_2879371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP38935
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.

实验方案

Product Specific Protocols
WB protocol for IGHMBP2 antibody 23945-1-APDownload protocol
IHC protocol for IGHMBP2 antibody 23945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

IGHMBP2 deletion suppresses translation and activates the integrated stress response

Authors - Jesslyn E Park
View Article