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验证数据展示

  • WB analysis using 23945-1-AP

    WB analysis using 23945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23945-1-AP

    IHC staining of human liver cancer using 23945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with IGHMBP2 antibody included

IGHMBP2 Polyclonal antibody

IGHMBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23945-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IGHMBP2 Ready-To-Use IHC Kit. IGHMBP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23945-1-AP targets IGHMBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 IGHMBP2 fusion protein Ag21057 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin mu binding protein 2
别名 ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2
计算分子量 993 aa, 109 kDa
观测分子量 109 kDa
GenBank蛋白编号BC105088
基因名称 IGHMBP2
Gene ID (NCBI) 3508
RRIDAB_2879371
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP38935
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.

实验方案

Product Specific Protocols
WB protocol for IGHMBP2 antibody 23945-1-APDownload protocol
IHC protocol for IGHMBP2 antibody 23945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

IGHMBP2 deletion suppresses translation and activates the integrated stress response

Authors - Jesslyn E Park
View Article