IGJ Polyclonal antibody
IGJ Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, mouse spleen tissue, human plasma |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IGJ Ready-To-Use IHC Kit. IGJ primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
13688-1-AP targets IGJ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Immunogen | IGJ fusion protein Ag4559 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
| Synonyms | IGCJ, IGJ, Immunoglobulin J chain, JCH |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC038982 |
| Gene Symbol | IGJ |
| Gene ID (NCBI) | 3512 |
| RRID | AB_2877973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IGJ antibody 13688-1-AP | Download protocol |
| IHC protocol for IGJ antibody 13688-1-AP | Download protocol |
| IP protocol for IGJ antibody 13688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. | ||
Int Immunopharmacol Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer. | ||
J Biotechnol Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells. | ||
Plant Mol Biol Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA. |
