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验证数据展示

  • IHC staining of human tonsillitis using 11781-1-AP

    IHC staining of human tonsillitis using 11781-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11781-1-AP (IgG Kappa Light Chain antibody) at dilution of 1:1000 (under 4x lens). heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 11781-1-AP

    WB analysis of human plasma using 11781-1-AP

    human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue, A549 cells, K-562 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11781-1-AP targets IgG Kappa Light Chain in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 IgG Kappa Light Chain fusion protein Ag2406 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin kappa variable 1-5
别名 IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1
计算分子量 239 aa, 26 kDa
观测分子量 26 kDa, 13 kDa
GenBank蛋白编号BC030814
基因名称 IgG Kappa Light Chain
Gene ID (NCBI) 28299
RRIDAB_10953529
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the kappa light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols