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IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11781-1-AP

Print datasheet

Synonyms

IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1

验证数据展示

  • IHC staining of human tonsillitis using 11781-1-AP

    IHC staining of human tonsillitis using 11781-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11781-1-AP (IgG Kappa Light Chain antibody) at dilution of 1:1000 (under 4x lens). heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 11781-1-AP

    WB analysis of human plasma using 11781-1-AP

    human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma tissue, A549 cells, K-562 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11781-1-AP targets IgG Kappa Light Chain in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IgG Kappa Light Chain fusion protein Ag2406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin kappa variable 1-5
Synonyms IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1
Calculated Molecular Weight 239 aa, 26 kDa
Observed Molecular Weight 26 kDa, 13 kDa
GenBank Accession NumberBC030814
Gene Symbol IgG Kappa Light Chain
Gene ID (NCBI) 28299
RRIDAB_10953529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the kappa light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols