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IgG Lambda Light Chain Polyclonal antibody

IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11541-1-AP

Print datasheet

Synonyms

IGL, immunoglobulin lambda locus, IGL-

验证数据展示

  • IHC staining of human tonsillitis using 11541-1-AP

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 11541-1-AP

    IF Staining of human tonsillitis using 11541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IgG Lambda Light Chain antibody (11541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human plasma using 11541-1-AP

    WB analysis of human plasma using 11541-1-AP

    human plasma (0.3 ug) were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Ramos using 11541-1-AP

    FC experiment of Ramos using 11541-1-AP

    1x10^6 Ramos cells were intracellularly stained with 0.4 ug IgG Lambda Light Chain Polyclonal antibody (11541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman plasma tissue, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC (Intra) detected inRamos cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11541-1-AP targets IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen IgG Lambda Light Chain fusion protein Ag2064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin lambda locus
Synonyms IGL, immunoglobulin lambda locus, IGL-
Calculated Molecular Weight 237 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC020233
Gene Symbol IgG Lambda Light Chain
Gene ID (NCBI) 3535
RRIDAB_2877775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the lambda light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
IHC protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
IF protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols