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验证数据展示

  • WB analysis of human brain using 14003-1-AP

    WB analysis of human brain using 14003-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14003-1-AP (IGSF11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14003-1-AP

    IHC staining of human kidney using 14003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 14003-1-AP

    IHC staining of human kidney using 14003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

IGSF11 Polyclonal antibody

IGSF11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14003-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

BT IgSF, BTIGSF, CXADRL1, IGSF11, Igsf13, VSIG3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14003-1-AP targets IGSF11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 IGSF11 fusion protein Ag5094 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin superfamily, member 11
别名 BT IgSF, BTIGSF, CXADRL1, IGSF11, Igsf13, VSIG3
计算分子量 430 aa, 46 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC034411
基因名称 IGSF11
Gene ID (NCBI) 152404
RRIDAB_10603368
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5DX21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IGSF11 antibody 14003-1-APDownload protocol
IHC protocol for IGSF11 antibody 14003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Neurosci Lett

Promotion of differentiation in developing mouse cerebellar granule cells by a cell adhesion molecule BT-IgSF.

Authors - Kasumi Higashine
  • KD Validated
View Article
humanIHC

Medicine (Baltimore)

Prediction of lung squamous cell carcinoma immune microenvironment and immunotherapy efficiency with pyroptosis-derived genes

Authors - Xiaheng Deng
View Article