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IGSF2 Monoclonal antibody, PBS Only

IGSF2 Monoclonal Antibody for WB,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

1E6A7

Cat No : 66333-1-PBS

Print datasheet

Synonyms

CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7

验证数据展示

  • FC experiment of RAW 264.7 using 66333-1-Ig (same clone as 66333-1-PBS)

    FC experiment of RAW 264.7 using 66333-1-Ig (same clone as 66333-1-PBS)

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.2 ug Anti-Human IGSF2 (66333-1-Ig, Clone:1E6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 66333-1-Ig (same clone as 66333-1-PBS)

    WB analysis of RAW 264.7 using 66333-1-Ig (same clone as 66333-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

    IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

    IHC staining of human lung cancer using 66333-1-Ig (same clone as 66333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66333-1-PBS in a different storage buffer formulation.

产品信息

66333-1-PBS targets IGSF2 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,FC Application Description
Tested Reactivity human, mouse
Immunogen IGSF2 fusion protein Ag23297 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin superfamily, member 2
Synonyms CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC136484
Gene Symbol IGSF2
Gene ID (NCBI) 9398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93033
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.