验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, U-118 MG cells, U-251 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18072-1-AP targets IGSF3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | IGSF3 fusion protein Ag12711 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin superfamily, member 3 |
| 别名 | V8, KIAA0466, Glu-Trp-Ile EWI motif-containing protein 3, EWI-3, EWI3 |
| 计算分子量 | 1195 aa, 135 kDa |
| 观测分子量 | 135-140 kDa |
| GenBank蛋白编号 | BC110651 |
| 基因名称 | IGSF3 |
| Gene ID (NCBI) | 3321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75054 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGSF3, known as EWI3, is a member of the immunoglobulin superfamily (IgSF) involved in various cell-cell interactions. IGSF3 is part of a cell adhesion molecule that is involved in cell-to-cell recognition, binding, and signal transduction. IGSF3 plays an important role in neural development, especially in the migration of neural crest cells and the development of the enteric nervous system. (PMID: 9541533; PMID: 37821496).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IGSF3 antibody 18072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |