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验证数据展示

  • WB analysis of K-562 using 14387-1-AP

    WB analysis of K-562 using 14387-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14387-1-AP

    IP experiment of mouse brain using 14387-1-AP

    IP result of anti-IGSF8/CD316 (IP:14387-1-AP, 3ug; Detection:14387-1-AP 1:500) with mouse brain tissue lysate 5000ug.

IGSF8/CD316 Polyclonal antibody

IGSF8/CD316 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14387-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14387-1-AP targets IGSF8/CD316 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 IGSF8/CD316 fusion protein Ag5846 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin superfamily, member 8
别名 CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL
计算分子量 65 kDa
观测分子量 70 kDa
GenBank蛋白编号BC053881
基因名称 IGSF8
Gene ID (NCBI) 93185
RRIDAB_2121881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969P0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

实验方案

Product Specific Protocols
WB protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
IP protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Ther Nucleic Acids

Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.

Authors - Ming-Jie Kuang
View Article
ratWB

Biochem Biophys Res Commun

Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.

Authors - Bao-Cheng Yang
View Article