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IGSF8/CD316 Polyclonal antibody

IGSF8/CD316 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14387-1-AP

Print datasheet

Synonyms

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL

验证数据展示

  • WB analysis of K-562 using 14387-1-AP

    WB analysis of K-562 using 14387-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 14387-1-AP

    IP experiment of mouse brain using 14387-1-AP

    IP result of anti-IGSF8/CD316 (IP:14387-1-AP, 3ug; Detection:14387-1-AP 1:500) with mouse brain tissue lysate 5000ug.

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14387-1-AP targets IGSF8/CD316 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen IGSF8/CD316 fusion protein Ag5846 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin superfamily, member 8
Synonyms CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC053881
Gene Symbol IGSF8
Gene ID (NCBI) 93185
RRIDAB_2121881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969P0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

实验方案

Product Specific Protocols
WB protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
IP protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Ther Nucleic Acids

Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.

Authors - Ming-Jie Kuang
View Article
ratWB

Biochem Biophys Res Commun

Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.

Authors - Bao-Cheng Yang
View Article