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IHCeasy ALPL Ready-To-Use IHC Kit

ALPL Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC2160

Synonyms

Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human lung tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using KHC2160 (ALPL IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC2160 (ALPL IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using KHC2160 (ALPL IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC2160 (ALPL IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using KHC2160 (ALPL IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using KHC2160 (ALPL IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using KHC2160 (ALPL IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC2160 is a ready-to-use IHC kit for staining of ALPL. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy ALPL Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI).

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP