IHCeasy BUB1B Ready-To-Use IHC Kit

BUB1B Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC2099

Synonyms

Bub1A, BUB1B, BUB1beta, BubR1, hBUBR1, MAD3L, Protein SSK1, SSK1



Product Information

KHC2099 is a ready-to-use IHC kit for staining of BUB1B. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy BUB1B Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells. In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation. Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms Bub1A, BUB1B, BUB1beta, BubR1, hBUBR1, MAD3L, Protein SSK1, SSK1