IHCeasy DUSP2 Ready-To-Use IHC Kit
DUSP2 Ready-to-use reagent kit for IHC.
Reactivity
Human, Rat
Sample Type
FFPE tissue
Cat No : KHC2394
Synonyms
PAC1, PAC 1, EC:3.1.3.16, Dual specificity protein phosphatase PAC-1, dual specificity phosphatase 2
Validation Data Gallery
Product Information
KHC2394 is a ready-to-use IHC kit for staining of DUSP2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy DUSP2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
DUSP2 belongs to the sub-family of nuclear specific DUSPs that includes DUSP1, 4 and 5. DUSP2 is highly expressed in immune cells, in particular, of patients with inflammatory arthritis, such as in rheumatoid arthritis (RA), and Dusp2 knockout mice present a significant reduction in inflammatory responses. It has been demonstrated that DUSP2 is a downstream target of the tumor suppressor p53 that is required for the cellular response to oxidative stress. DUSP2 is also involved in transcription factor E2F1-mediated apoptotic signaling. Like other members of the DUSP family, DUSP2 interacts with MAPKs; However, the target protein for its phosphatase activity is controversial.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | PAC1, PAC 1, EC:3.1.3.16, Dual specificity protein phosphatase PAC-1, dual specificity phosphatase 2 |