IHCeasy LIS1 Ready-To-Use IHC Kit
LIS1 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC2272
Synonyms
PAFAH1B1, PAF-AH alpha, PAF-AH 45 kDa subunit, Lissencephaly-1 protein, LIS-1
Validation Data Gallery
Product Information
KHC2272 is a ready-to-use IHC kit for staining of LIS1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy LIS1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | PAFAH1B1, PAF-AH alpha, PAF-AH 45 kDa subunit, Lissencephaly-1 protein, LIS-1 |