IHCeasy LPCAT2 Ready-To-Use IHC Kit
LPCAT2 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC2615
Synonyms
LPCAT2,1-acylglycerol-3-phosphate O-acyltransferas
Validation Data Gallery
Product Information
KHC2615 is a ready-to-use IHC kit for staining of LPCAT2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy LPCAT2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
LPCAT2 (Lysophosphatidylcholine acyltransferase 2) is also named as AGPAT11 and AYTL1. Lipid droplet (LD) content of CRC cells positively correlates with the expression of lysophosphatidylcholine acyltransferase 2 (LPCAT2), an LD-localised enzyme supporting phosphatidylcholine synthesis. Mitochondria play a significant role in ACSL4/LPCAT2-driven ferroptosis. In response to LPS, LPCAT2, but not LPCAT1, rapidly associates with TLR4 and translocates to membrane lipid raft domains to regulate macrophage inflammatory gene expression.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | LPCAT2,1-acylglycerol-3-phosphate O-acyltransferas |
