IHCeasy MAPKAPK2 Ready-To-Use IHC Kit
MAPKAPK2 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC2347
Synonyms
MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2
Validation Data Gallery
Product Information
KHC2347 is a ready-to-use IHC kit for staining of MAPKAPK2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy MAPKAPK2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation. It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2 |