IHCeasy PKIA Ready-To-Use IHC Kit
PKIA Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC2343
Synonyms
PKIA,cAMP-dependent protein kinase inhibitor alpha
Validation Data Gallery
Product Information
KHC2343 is a ready-to-use IHC kit for staining of PKIA. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PKIA Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals. Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores. miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | PKIA,cAMP-dependent protein kinase inhibitor alpha |
