IHCeasy PRKAB2 Ready-To-Use IHC Kit

PRKAB2 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC2093

Synonyms

AMPK beta 2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2



Product Information

KHC2093 is a ready-to-use IHC kit for staining of PRKAB2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy PRKAB2 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms AMPK beta 2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2