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IHCeasy PRKCQ Ready-To-Use IHC Kit

PRKCQ Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse

Sample Type

FFPE tissue

Cat No : KHC2392

Synonyms

Protein kinase C theta type, protein kinase C, PRKCT, nPKC-theta, EC:2.7.11.13

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC2392 (PRKCQ IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC2392 (PRKCQ IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using KHC2392 (PRKCQ IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using KHC2392 (PRKCQ IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC2392 is a ready-to-use IHC kit for staining of PRKCQ. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy PRKCQ Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms Protein kinase C theta type, protein kinase C, PRKCT, nPKC-theta, EC:2.7.11.13