IHCeasy RAD17 Ready-To-Use IHC Kit
RAD17 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC2123
Synonyms
CCYC, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog
Validation Data Gallery
Product Information
KHC2123 is a ready-to-use IHC kit for staining of RAD17. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy RAD17 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Rad17, a component of the checkpoint clamp loader complex (RAD17/RFC), was originally initially identified as a DNA damage checkpoint protein. Rad17 participates in cell cycle control and DNA damage repair. Rad17 phosphorylation by ATR (ataxia telangiectasia mutated and Rad3-related) kinase is indispensable for the recruitment of Claspin and the activation of Chk1 in response to genotoxic stress.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | CCYC, HRAD17, R24L, RAD17, RAD17 homolog (S. pombe), RAD17SP, RAD24, RF C/activator 1 homolog |
