IHCeasy RBM15 Ready-To-Use IHC Kit

RBM15 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0150

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN



Product Information

KHC0150 is a ready-to-use IHC kit for staining of RBM15. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy RBM15 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN