IHH Polyclonal antibody
IHH Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human kidney tissue, HEK-293 cells, human heart tissue |
Positive IHC detected in | human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13388-1-AP targets IHH in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | IHH fusion protein Ag4046 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Indian hedgehog homolog (Drosophila) |
Synonyms | Indian hedgehog protein N-product, HHG-2, HHG2, HHG 2, EC:3.1.-.- |
Calculated Molecular Weight | 411 aa, 45 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC034757 |
Gene Symbol | IHH |
Gene ID (NCBI) | 3549 |
RRID | AB_2248725 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14623 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for IHH antibody 13388-1-AP | Download protocol |
IHC protocol for IHH antibody 13388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
Cell Mol Life Sci Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway. | ||
Mol Ther Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2. | ||
Osteoarthritis Cartilage mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis. | ||
Int J Mol Sci Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism. | ||
Dev Biol DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development. |