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IIP45 Polyclonal antibody

IIP45 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20630-1-AP

Print datasheet

Synonyms

IGFBP2 binding protein, IIP45, invasion inhibitory protein 45, MIIP

验证数据展示

  • IHC staining of human liver using 20630-1-AP

    IHC staining of human liver using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 20630-1-AP

    IHC staining of human liver using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of fetal human brain using 20630-1-AP

    WB analysis of fetal human brain using 20630-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20630-1-AP (IIP45 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20630-1-AP is IIP45 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen IIP45 fusion protein Ag14609 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name invasion inhibitory protein 45
Synonyms IGFBP2 binding protein, IIP45, invasion inhibitory protein 45, MIIP
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008068
Gene Symbol IIP45
Gene ID (NCBI) 60672
RRIDAB_2878712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JXC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IIP45 antibody 20630-1-APDownload protocol
IHC protocol for IIP45 antibody 20630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

GAS5 suppresses malignancy of human glioma stem cells via a miR-196a-5p/FOXO1 feedback loop.

Authors - Xihe Zhao
View Article
humanIHC

Cancer Biol Med

MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis

Authors - Fengqi Yan
View Article
mouseIHC,WB

Appl Microbiol Biotechnol

VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway

Authors - Qian Wang
View Article