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验证数据展示

  • WB analysis using 31833-1-AP

    WB analysis using 31833-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31833-1-AP

    IHC staining of mouse testis using 31833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 31833-1-AP

    IF Staining of A431 using 31833-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

IK Polyclonal antibody

IK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31833-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

RED, Protein RER, Protein Red, IK factor, IK Cytokine

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 IK fusion protein Ag37095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IK cytokine, down-regulator of HLA II
别名 RED, Protein RER, Protein Red, IK factor, IK Cytokine
计算分子量 557 aa, 66 kDa
观测分子量70 kDa
GenBank蛋白编号BC013005
基因名称 IK
Gene ID (NCBI) 3550
RRIDAB_3670123
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13123
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.

实验方案

Product Specific Protocols
WB protocol for IK antibody 31833-1-APDownload protocol
IHC protocol for IK antibody 31833-1-APDownload protocol
IF protocol for IK antibody 31833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols