验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | DU 145 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
20979-1-AP targets IKBKB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta |
| 别名 | I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB |
| 计算分子量 | 87 kDa |
| 观测分子量 | 80 kDa, 86 kDa, 87 kDa |
| GenBank蛋白编号 | NM_001556 |
| 基因名称 | IKBKB |
| Gene ID (NCBI) | 3551 |
| RRID | AB_2878780 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14920 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IKBKB, also named as NFKBIKB, IKK2 and IKKB, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It also phosphorylates NCOA3. The antibody is specific to IKBKB. This antibody can identify 3 isoform of IKBKB with the molecular weight of 80, 86, 87 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IKBKB antibody 20979-1-AP | Download protocol |
| IHC protocol for IKBKB antibody 20979-1-AP | Download protocol |
| IF protocol for IKBKB antibody 20979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. | ||
Neurochem Res Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1(G93A) ALS Mice. |
