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IKIP Polyclonal antibody

IKIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14589-1-AP

Print datasheet

Synonyms

IKBIP, IKBKB interacting protein, IKIP, IKK interacting protein

验证数据展示

  • WB analysis of mouse skeletal muscle using 14589-1-AP

    WB analysis of mouse skeletal muscle using 14589-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 14589-1-AP

    IHC staining of human ovary tumor using 14589-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14589-1-AP

    IHC staining of human ovary tumor using 14589-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14589-1-AP targets IKIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen IKIP fusion protein Ag6119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IKK interacting protein
Synonyms IKBIP, IKBKB interacting protein, IKIP, IKK interacting protein
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC058933
Gene Symbol IKIP
Gene ID (NCBI) 121457
RRIDAB_1557451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70UQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKIP(Inhibitor of nuclear factor kappa-B kinase-interacting protein) is also named as IKBIP and acts as a novel p53 target gene with proapoptotic function. A variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining. EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins(PMID:15389287).

实验方案

Product Specific Protocols
WB protocol for IKIP antibody 14589-1-APDownload protocol
IHC protocol for IKIP antibody 14589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

FASEB J

WNT2B activates macrophages via NF-κB signaling pathway in inflammatory bowel disease

Authors - Lin Lan
View Article
humanWB

Oncol Res

IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells

Authors - Zhaoying Zhu
View Article