验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14589-1-AP targets IKIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | IKIP fusion protein Ag6119 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | IKK interacting protein |
| Synonyms | IKBIP, IKBKB interacting protein, IKIP, IKK interacting protein |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC058933 |
| Gene Symbol | IKIP |
| Gene ID (NCBI) | 121457 |
| RRID | AB_1557451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70UQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IKIP(Inhibitor of nuclear factor kappa-B kinase-interacting protein) is also named as IKBIP and acts as a novel p53 target gene with proapoptotic function. A variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining. EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins(PMID:15389287).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IKIP antibody 14589-1-AP | Download protocol |
| IHC protocol for IKIP antibody 14589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
