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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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IKKA Recombinant antibody, PBS Only

IKKA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241579D12

Cat no : 84372-5-PBS

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HCT 116 using 84372-5-RR (same clone as 84372-5-PBS)

    IF Staining of HCT 116 using 84372-5-RR (same clone as 84372-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using IKKA antibody (84372-5-RR, Clone: 241579D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84372-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84372-5-RR (same clone as 84372-5-PBS)

    FC experiment of HepG2 using 84372-5-RR (same clone as 84372-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant Antibody (84372-5-RR, Clone:241579D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84372-5-PBS in a different storage buffer formulation.

产品信息

The immunogen of 84372-5-PBS is IKKA Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen IKKA fusion protein Ag26573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name conserved helix-loop-helix ubiquitous kinase
Synonyms
Calculated Molecular Weight 85 kDa
GenBank Accession NumberNM_001278
Gene Symbol IKK Alpha
Gene ID (NCBI) 1147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15111
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NF‐κB is regulated by phosphorylation of one of its cytoplasmic inhibitors, IκB, mediated by IκB kinases (IKK), allowing NF‐κB nuclear translocation and transcriptional activation. IKKα‐IKKβ heterodimer complexes regulate the DNA binding proteins implicated in the canonical NF‐κB pathway, including p50‐p65, while IKKα‐homodimers regulate p52‐RELB dimers implicated in the alternative NF‐κB pathway. (PMID: 34187082)