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IKKa antibody (mAb) (Clone 14A231)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB

CloneNo.

14A231

Cat No : 40905 40905

Synonyms

IKKa, IKK-a, IKK a, IK Ka, IK K-a, IKKalpha, IKK-alpha, IKK alpha, mAb, monoclonal, antibody, antibodies, nfkb

验证数据展示

  • IKKa mAb tested by Western blot. IKKa detection by Western blot analysis. The detection of IKKa was performed using 30 ug of whole-cell extract from Daudi cells with the IKKa mAb at a 1 ug/ml dilution.

    IKKa mAb tested by Western blot. IKKa detection by Western blot analysis. The detection of IKKa was performed using 30 ug of whole-cell extract from Daudi cells with the IKKa mAb at a 1 ug/ml dilution.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This IKKa antibody was raised against a full-length protein corresponding to human IKKa.
Full Name IKKa antibody (mAb) (Clone 14A231)
Synonyms IKKa, IKK-a, IKK a, IK Ka, IK K-a, IKKalpha, IKK-alpha, IKK alpha, mAb, monoclonal, antibody, antibodies, nfkb
Molecular weight 85 kDa
GenBank accession numberNP_001269
RRIDAB_2793428
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

IKKa (IkB Kinase alpha subunit) – The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa.